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The 2nd reaction cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to produce ferric enzyme, NO, citrulline, and water as products (Fig. 9) - circular dichroism. As attracted Fig - https://allmyfaves.com/olisclarity1?tab=Olis%20Clarity. 9, the reaction may also include electron transfer from H4B to the Fe, IIO2 intermediate
l9 As shown in Fig. 9, this could be because the H4B radical acts as an electron sink in a later point in the response, so as to enable NOS to, Fig. 9. A model for NORA oxidation by NOSoxy in the single turnover response. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that reacts with NOHA.